Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru the bacterial growth ttest. Your comments are invited either for revision of this test method or for additional methods and should be addressed to the AATCC Technical Center.
The average width of a zone of inhibition along a streak on either side of the test specimen may be calculated using the following equation: AATCC takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this test method.
The test is followed in the cases where the antibacterial agent is migratory i. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.
AATCC Test Method 147
To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the contact area. Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment.
Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity.
Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend. Therefore, every necessary and 5. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram trst organism.
Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. At the conclusion of two weeks, make a fresh transplant from stock culture. Peptone Bacto-peptone see Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see AATCC grants you a license as follows: Avoid contamination by using good sterile technique in plating and transferring.
The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degrees of sensitivity. Maintenance of Culture of Test Organisms 8. Wear safety glasses in all laboratory areas. Take care not to break the surface of the agar while making the streaks.
Antimicrobial – Parallel Streak Method AATCC 147
After incubation, a clear 14 of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Plug and sterilize at kPa 15 psi for 15 minutes. Uses and Limitations 1. In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test methood and contaminant organisms which may be present on the unsterilized specimen.
Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need.
BoxManassas VA ; tel: The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings.
Check culture purity by making streak plates periodically and observing for a single species-characteristic type msthod colonies. Mix well using appropriate agitation.
May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this.
All OSHA standards and rules must also be consulted and followed. In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise. The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
The criterion for passing the test must be agreed upon by the interested parties. Allow agar to gel firmly before aarcc.
If you feel that your comments have not received a fair hearing, you should make your views known methos Christopher S.
AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.
Rectangular specimens cut 25 – 50 mm They can be any convenient size. Report of results will include an observation of zones of inhibition and growth under the specimen if present. Precision and Bias 7. The hard copy print may only be distributed to other employees for their internal use within your organization. This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or withdrawn.
The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings. Other recommended strains are listed below in Section 6. A standard strain of bacteria is used which is specific to the requirements of the material under test.
This test method is not for resale. Adjust to pH 6.
After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Specimens of the test material, including corresponding untreated controls tesg the same material, are placed in intimate contact with growth agar which has been previously streaked with test organism.